Portuino—A Novel Portable Low-Cost Arduino-Based Photo- and Fluorimeter

  • A novel portable low-cost Arduino-controlled photo- and fluorimeter for on-site measurements has been developed. The device uses LEDs as a light source and a phototransistor as a light sensor. The circuit is based on the discharge of a capacitor with the photocurrent from the phototransistor. Validation experiments for absorbance measurements were performed by measuring protein concentration using the Bradford method and measuring phosphate ions in water using a commercial test kit. The emission light of the excited fluorescent dyes rhodamine 6G and calcofluor white was measured to validate the usability of the device as a fluorescence photometer. In all validation experiments, similar correlation coefficients and limit of detection could be achieved with the portable photo- and fluorimeter and a laboratory spectrometer and fluorimeter. Real sample analysis was performed, measuring phosphate concentration in freshwater and concentration of green fluorescent protein, extracted from Escherichia coli.

Download full text files

Export metadata

Author:Sarah Di Nonno, Roland Ulber
Parent Title (English):Sensors
Document Type:Article
Language of publication:English
Publication Date:2022/10/18
Year of Publication:2022
Publishing Institute:Technische Universität Kaiserslautern
Date of the Publication (Server):2022/12/02
Issue:2022, 22, 7916
Number of page:13
Faculties / Organisational entities:Kaiserslautern - Fachbereich Maschinenbau und Verfahrenstechnik
DDC-Cassification:6 Technik, Medizin, angewandte Wissenschaften / 620 Ingenieurwissenschaften und Maschinenbau
Licence (German):Zweitveröffentlichung