Insight into Fucoidan-based PEGylated PLGA Nanoparticles Encapsulating Methyl Anthranilic Acid: In Vitro Evaluation and In Vivo Anti-inflammatory Study

  • A potential fucoidan-based PEGylated PLGA nanoparticles (NPs) offering a proper delivery of N-methyl anthranilic acid (MA, a model of hydrophobic anti-inflammatory drug) have been developed via the formation of fucoidan aqueous coating surrounding PEGylated PLGA NPs. The optimum formulation (FuP2) composed of fucoidan:m-PEG-PLGA (1:0.5 w/w) with particle size(365 ± 20.76 nm), zeta potential (-22.30 ± 2.56 mV), % entrapment efficiency (85.45 ± 7.41), drug loading (51.36 ± 4.75 µg/mg of NPs), % initial burst (47.91 ± 5.89), and % cumulative release (102.79 ± 6.89) has been further investigated for the anti-inflammatory in vivo study. This effect of FuP2 was assessed in rats’ carrageenan-induced acute inflammation model. The average weight of the paw edema was significantly lowered (p ≤ 0.05) by treatment with FuP2. Moreover, cyclooxygenase-2 and tumor necrosis factor-alpha immunostaining were decreased in FuP2 treated group compared to the other groups. The levels of prostaglandin E2, nitric oxide, and malondialdehyde were significantly reduced (p ≤ 0.05) in the FuP2-treated group. A significant reduction (p ≤ 0.05) in the expression of interleukins (IL-1b and IL-6) with an improvement of the histological findings of the paw tissues was observed in the FuP2-treated group. Thus, fucoidan-based PEGylated PLGA–MA NPs are a promising anti-inflammatory delivery system that can be applied for other similar drugs potentiating their pharmacological and pharmacokinetic properties.

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Author:Dalia AbdelkaderORCiD, Engy ElekhnawyORCiD, Walaa NegmORCiD, Thanaa El-Masry, May AlmukainziORCiD, Ahmed ZayedORCiD
Parent Title (English):Marine Drugs
Place of publication:Basel, Switzerland
Editor(s):Marc DiederichORCiD
Document Type:Article
Language of publication:English
Publication Date:2022/11/04
Year of Publication:2022
Publishing Institute:Technische Universität Kaiserslautern
Date of the Publication (Server):2022/12/05
Tag:aqueous coating; cyclooxygenase-2; interleukins; reactive oxygen species; single emulsion solvent evaporation; tumor necrosis factor-alpha
Issue:2022, 20(11), 694
Number of page:19
Faculties / Organisational entities:Kaiserslautern - Fachbereich Maschinenbau und Verfahrenstechnik
DDC-Cassification:6 Technik, Medizin, angewandte Wissenschaften / 620 Ingenieurwissenschaften und Maschinenbau
Licence (German):Zweitveröffentlichung