In vivo and in vitro studies on heavy doses and fluorescent-labelled vitamin C using ESR and fluorescence spectroscopy

  • Under physiological conditions oxygen is constantly being converted to reactive oxygen intermediates, in mitochondria, peroxisomes, cytochrome p450 systems, macrophages, neutrophils and in plasma membranes. These reactive oxygen species (ROS) are toxic and therefore alter cell integrity leading to cell damage. To protect itself against this toxic effect of ROS, living systems have developed defence systems that scavenge ROS formation. These systems include some enzymes, transporting proteins and small antioxidant molecules for instance vitamin C and E. This thesis describes a study on the antioxidant chemistry and activity of vitamin C in vivo and in vitro systems using ESR spectroscopy. Also, a new method was designed to label ascorbic acid with a fluorescent marker. Moreover, some important criteria were considered for the evaluation and quantification of ascorbyl radicals in human blood plasma using two types of ESR spectrometers.
  • In-vivo- und in-vitro-Untersuchungen über hoch dosiertes und Fluoreszenz-markiertes Vitamin C mit ESR- und Fluoreszenzspektroskopie

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Author:Fabian Rozario
Advisor:Wolfgang Trommer
Document Type:Doctoral Thesis
Language of publication:English
Year of Completion:2005
Year of Publication:2005
Publishing Institute:Technische Universität Kaiserslautern
Granting Institute:Technische Universität Kaiserslautern
Acceptance Date of the Thesis:2005/02/22
Date of the Publication (Server):2005/04/05
Tag:Ascorbat; Ascorbinsäure; Ascorbylradikal; EPR; ESR; Vitamin C
EPR; ESR; Vitamin C; ascorbate; ascorbic acid; ascorbyl radical
GND-Keyword:Vitamin C; Vitamin C-Derivate; Elektronenspinresonanz
Faculties / Organisational entities:Kaiserslautern - Fachbereich Chemie
DDC-Cassification:5 Naturwissenschaften und Mathematik / 540 Chemie
Licence (German):Standard gemäß KLUEDO-Leitlinien vor dem 27.05.2011