A New UPLC-qTOF Approach for Elucidating Furan and 2-Methylfuran Metabolites in Human Urine Samples after Coffee Consumption
- We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine-cis-2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies.
Verfasser*innenangaben: | Simone Stegmüller, Nadine Beißmann, Jonathan Isaak KremerORCiD, Denise Mehl, Christian Baumann, Elke RichlingORCiD |
---|---|
URN: | urn:nbn:de:hbz:386-kluedo-61797 |
ISSN: | 1420-3049 |
Titel des übergeordneten Werkes (Englisch): | Molecules |
Verlag: | MDPI |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache der Veröffentlichung: | Englisch |
Datum der Veröffentlichung (online): | 03.11.2020 |
Jahr der Erstveröffentlichung: | 2020 |
Veröffentlichende Institution: | Technische Universität Kaiserslautern |
Datum der Publikation (Server): | 14.01.2021 |
Ausgabe / Heft: | (2020) 25 |
Seitenzahl: | 14 |
Quelle: | https://www.mdpi.com/1420-3049/25/21/5104 |
Fachbereiche / Organisatorische Einheiten: | Kaiserslautern - Fachbereich Chemie |
DDC-Sachgruppen: | 5 Naturwissenschaften und Mathematik / 540 Chemie |
Sammlungen: | Open-Access-Publikationsfonds |
Lizenz (Deutsch): | Zweitveröffentlichung |