Thanh Chien Nguyen

• Field-effect transistor (FET) sensors and in particular their nanoscale variant of silicon nanowire transistors are very promising technology platforms for label-free biosensor applications. These devices directly detect the intrinsic electrical charge of biomolecules at the sensor’s liquid-solid interface. The maturity of micro fabrication techniques enables very large FET sensor arrays for massive multiplex detection. However, the direct detection of charged molecules in liquids faces a significant limitation due to a charge screening effect in physiological solutions, which inhibits the realization of point-of-care applications. As an alternative, impedance spectroscopy with FET devices has the potential to enable measurements in physiological samples. Even though promising studies were published in the field, impedimetric detection with silicon FET devices is not well understood. The first goal of this thesis was to understand the device performances and to relate the effects seen in biosensing experiments to device and biomolecule types. A model approach should help to understand the capability and limitations of the impedimetric measurement method with FET biosensors. In addition, to obtain experimental results, a high precision readout device was needed. Consequently, the second goal was to build up multi-channel, highly accurate amplifier systems that would also enable future multi-parameter handheld devices. A PSPICE FET model for potentiometric and impedimetric detection was adapted to the experiments and further expanded to investigate the sensing mechanism, the working principle, and effects of side parameters for the biosensor experiments. For potentiometric experiments, the pH sensitivity of the sensors was also included in this modelling approach. For impedimetric experiments, solutions of different conductivity were used to validate the suggested theories and assumptions. The impedance spectra showed two pronounced frequency domains: a low-pass characteristic at lower frequencies and a resonance effect at higher frequencies. The former can be interpreted as a contribution of the source and double layer capacitances. The latter can be interpreted as a combined effect of the drain capacitance with the operational amplifier in the transimpedance circuit. Two readout systems, one as a laboratory system and one as a point-of-care demonstrator, were developed and used for several chemical and biosensing experiments. The PSPICE model applied to the sensors and circuits were utilized to optimize the systems and to explain the sensor responses. The systems as well as the developed modelling approach were a significant step towards portable instruments with combined transducer principles in future healthcare applications.